BioG 1105-1106 at Cornell University
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Unit 3: Demos

Objective 3:

Operon Tutorial
lac operon animation
Induction of the lac operon

Objectives 4 & 5:

Positive v. negative control of prokaryotic gene transcription

Objective 6:

Transcription of eukaryotic vs. prokaryotic genomes (6c)

Objective 7:

How Does Methylation Control Synthesis of Proteins?
You are what your grandmother ate - diet linked to methylation (optional)
Genetic Imprinting: The Battle of the Sexes - Reloaded (optional)
Genome biology: She moves in mysterious ways - The human X chromosome is a study in contradictions. The detailed sequence of the X, and a survey of inactivated genes in females, help to illuminate this unique 'evolutionary space'.
Unexpressed but Indispensable: The (non-coding) DNA Sequences that Control Development. (optional)

Objective 9:

optional:

Diagram showing how Dolly was made (from Liem et al. Functional Anatomy of the Vertebrates, 3rd ed.)
Give the dog a clone
ViaGen: Gene Banking and Cloning of Exceptional Pets

Objective 14:

Yolk content and cleavage in human eggs (14c)
Zygote and morula (14d):
Zygotes: sea urchin, frog (zygote upper left, morula bottom right)

Objective 16:

Notochord development (16a)
Neural crest cells (16d):
Formation and fate of neural crest cells (16d)
Scientists Hatch a Few Odd Birds  (optional)
Somite development (16e)

Objective 17:

Comparative development (17b)

Objectives 19 & 20:

Determinate v. indeterminate cleavage & embryonic induction

Slides

Unit 3 Slides (accompanying text can be found in your Survival Manual on pages 57-59.)

Notochord Development

The notochord is one of the defining characteristics of the phylum Chordata and serves as a internal support structure for the body, preventing telescoping of the body when lateral muscles contract. Present at some stage of development in all chordates, the notochord's role is taken over by the vertebral column in the adults of many vertebrate groups. Note that the notochord develops from the band of dorsally-positioned mesoderm that initially flows into the developing embryo during the early stages of gastrulation. The mesoderm that forms the notochord is called chordamesoderm and is important not only in forming the notochord itself but also in inducing development of the overlying ectoderm into the neural tube (see Figure 12.20 at bottom of page).

Figure 4.13 from K. Kalthoff. 2001. Analysis of Biological Development. McGraw Hill, Boston.

Notochord function

Figure 2.5 from K.V. Kardong. 2002. Vertebrates - Comparative Anatomy, Function, Evolution, 3rd edition. McGraw Hill, Boston.

Induction by the notochord / chordamesoderm

Figure 12.20 from K. Kalthoff. 2001. Analysis of Biological Development. McGraw Hill, Boston.

Experiments like the one shown in Figure 12.20 (above) demonstrated that the chordamesoderm (the tissue that initially forms the dorsal lip of the blastopore and then moves into the embryo later in gastrulation) is important in inducing the proper development of the overlying ectodermal tissue into the neural tube.

© BIOG 1105-1106