BIOG 1105-1106 | Cornell Introductory Biology, Individualized Instruction

BioG 1105-1106 at Cornell University

Unit 2: Demos

Objective 3:

Promoter strength (3c)
What Is The Role Of RNA Polymerase In Transcription? What are promoter sites?
RNA polymerase termination signal in prokaryotes (3d)
Zebrafish Glow in the Morning - firefly gene linked to biological clock promoter helps researchers understand circadian rhythms. (optional)

Objective 7:

Animation: Gene Information Processing in Prokaryotes vs. Eukaryotes ^(optional)

Objective 11:

Transposons: Spam from the Dark Ages ^(optional)

Objective 12:

Recombinant DNA and gene cloning
Give the dog a clone ^(optional)

Objective 13:

PCR (supplementary):

What the heck is PCR?
PCR schematic
Diagram of the PCR reactions

Objective 14:

Gel electrophoresis (supplementary):

Southern Blot Schematic

Objective 15:

Genome mapping (supplementary)

Objective 16:

DNA profiling (supplementary):

Saddam's DNA test a fast job
DNA profiling - Mallory, Lost on Everest (Nova Online)

Optional Supplementary Material:

Gene Therapy
RNA vs DNA
Origin of tRNA and rRNA

Genetic engineering:

Flower Power Takes on Land Mines (story with image)
Bacteria modified to help make missile fuel

The Origin of tRNA and rRNA

It should be evident that all types of RNA are transcribed from a DNA template. Although strands of transfer and ribosomal RNA are never translated into polypeptides, they are synthesized from nuclear (and mitochondrial) DNA in the same manner as messenger RNA. Yes, there are tRNA and rRNA genes! The latter are grouped into a mass called the “nucleolus,” which is often visible when stained. Roughly 300 rRNA and 1300 tRNA genes are known to exist in the human genome.

Diagram of tyrosine tRNA gene of Escherichia coli indicating transcription control elements on either side; promoter region to left, terminator region to right.

© BIOG 1105-1106

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