Cornell University BIOG 1105-1106
Unit 2: Demos

Objective 3:

Promoter strength (3c)
What Is The Role Of RNA Polymerase In Transcription? What are promoter sites?
RNA polymerase termination signal in prokaryotes (3d)
Zebrafish Glow in the Morning - firefly gene linked to biological clock promoter helps researchers understand circadian rhythms. (optional)

Objective 12:

Transposons: Spam from the Dark Ages (optional)

Objective 13:

Recombinant DNA and gene cloning
Give the dog a clone (optional)

Objective 14

PCR (supplementary):

What the heck is PCR?
Principle of PCR
Check out the book Making PCR by Paul Rabinow. Call # QP606.D46 R33x 1996 at either Olin or Uris.
PCR schematic
Diagram of the PCR reactions

Objective 15:

Gel electrophoresis (supplementary):

Southern Blot Schematic

Objective 16:

Genome mapping (supplementary)
A physical map of the human Y chromosome (optional)

Objective 17:

DNA profiling (supplementary):

Disclosure of variation - Now that the sequence of the human genome is almost complete, the human genomics community is turning its attention towards what, genetically speaking, makes people different.
Saddam's DNA test a fast job (Wired News)
DNA profiling - Mallory, Lost on Everest (Nova Online)
also see the BIOG 1105-1106 DNA Lab page

Optional Supplementary Material:

Gene Therapy
RNA vs DNA
Origin of tRNA and rRNA

Genetic engineering:

Flower Power Takes on Land Mines (story with image)
Bacteria modified to help make missile fuel
Molecular Biology Tutorials
In Situ Hybridization diagram

The Origin of tRNA and rRNA

It should be evident that all types of RNA are transcribed from a DNA template. Although strands of transfer and ribosomal RNA are never translated into polypeptides, they are synthesized from nuclear (and mitochondrial) DNA in the same manner as messenger RNA. Yes, there are tRNA and rRNA genes! The latter are grouped into a mass called the “nucleolus,” which is often visible when stained. Roughly 300 rRNA and 1300 tRNA genes are known to exist in the human genome.

Diagram of tyrosine tRNA gene of Escherichia coli indicating transcription control elements on either side; promoter region to left, terminator region to right.

© 2010 | BIOG 1105-1106