Unit 2: Information Transfer and Mobile Genetic Elements.

"Within each cell, genetic information flows from DNA to RNA to protein." - Francis Crick, explaining the (then new) central dogma of molecular biology in 1957.

Objective 3:

Promoter strength (3c)
What Is The Role Of RNA Polymerase In Transcription? What are promoter sites?
RNA polymerase termination signal in prokaryotes (3d)
Zebrafish Glow in the Morning - firefly gene linked to biological clock promoter helps researchers understand circadian rhythms. (optional)

Objective 12:

Transposons: Spam from the Dark Ages ^(optional)

Objective 13:

Recombinant DNA and gene cloning
Give the dog a clone ^(optional)

Objective 14

PCR (supplementary):

What the heck is PCR?
Principle of PCR
Check out the book Making PCR by Paul Rabinow. Call # QP606.D46 R33x 1996 at either Olin or Uris.
PCR schematic
Diagram of the PCR reactions

Objective 15:

Gel electrophoresis (supplementary):

Southern Blot Schematic

Objective 16:

Genome mapping (supplementary)
A physical map of the human Y chromosome (optional)

Objective 17:

DNA profiling (supplementary):

Disclosure of variation - Now that the sequence of the human genome is almost complete, the human genomics community is turning its attention towards what, genetically speaking, makes people different.
Saddam's DNA test a fast job (Wired News)
DNA profiling - Mallory, Lost on Everest (Nova Online)
also see the BIOG 1105-1106 DNA Lab page

Optional Supplementary Material:

Gene Therapy
RNA vs DNA
Origin of tRNA and rRNA

Genetic engineering:

Flower Power Takes on Land Mines (story with image)
Bacteria modified to help make missile fuel
Molecular Biology Tutorials
In Situ Hybridization diagram