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Objective 3:
Promoter strength (3c)
What Is The Role Of RNA Polymerase In Transcription? What are promoter
sites?
RNA polymerase termination
signal in prokaryotes (3d)
Zebrafish Glow in the Morning - firefly gene linked to biological clock
promoter helps researchers understand circadian rhythms. (optional)
Objective 12:
Transposons:
Spam from the Dark Ages (optional)
Objective 13:
Recombinant DNA and gene cloning
Give
the dog a clone (optional)
Objective 14
PCR (supplementary):
What the heck is PCR?
Principle of PCR
Check out the book Making
PCR by Paul Rabinow. Call # QP606.D46 R33x 1996 at either Olin or Uris.
PCR
schematic
Diagram of the PCR reactions
Objective 15:
Gel electrophoresis (supplementary):
Southern Blot Schematic
Objective 16:
Genome
mapping (supplementary)
A
physical map of the human Y chromosome (optional)
Objective 17:
DNA profiling (supplementary):
Disclosure
of variation - Now that the sequence of the human genome
is almost complete, the human genomics community is turning its
attention towards what, genetically speaking, makes people different.
Saddam's
DNA test a fast job (Wired News)
DNA
profiling - Mallory, Lost on Everest (Nova Online)
also see the BIOG 1105-1106 DNA Lab page
Optional Supplementary Material:
Gene Therapy
RNA vs DNA
Origin of tRNA
and rRNA
Genetic engineering:
Flower
Power Takes on Land Mines (story with image)
Bacteria
modified to help make missile fuel
Molecular
Biology Tutorials
In
Situ Hybridization diagram
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RNA vs DNA
Here are the ribonucleotides (left column) found in RNA and the
deoxyribonucleotides (right column) found in DNA. How do these two nucleotides
differ?
The building blocks of RNA and DNA. Each nucleotide building block contains
a phosphate group, a sugar, and a nitrogenous base, which can be either a purine
or a pyrimidine.
The purines in both groups are the same, but one type of DNA nucleotide
contains thymine, whereas its RNA counterpart contains uracil. The only other
difference
between the two is the presence of one more oxygen on the sugar (ribose)
component of the RNA. The biological roles of the two are profoundly different.
You must be able to distinguish between DNA nucleotides and RNA nucleotides.
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