Cornell University BIOG 1105-1106
Unit 2: Demos

Objective 3:

Promoter strength (3c)
What Is The Role Of RNA Polymerase In Transcription? What are promoter sites?
RNA polymerase termination signal in prokaryotes (3d)
Zebrafish Glow in the Morning - firefly gene linked to biological clock promoter helps researchers understand circadian rhythms. (optional)

Objective 12:

Transposons: Spam from the Dark Ages (optional)

Objective 13:

Recombinant DNA and gene cloning
Give the dog a clone (optional)

Objective 14

PCR (supplementary):

What the heck is PCR?
Principle of PCR
Check out the book Making PCR by Paul Rabinow. Call # QP606.D46 R33x 1996 at either Olin or Uris.
PCR schematic
Diagram of the PCR reactions

Objective 15:

Gel electrophoresis (supplementary):

Southern Blot Schematic

Objective 16:

Genome mapping (supplementary)
A physical map of the human Y chromosome (optional)

Objective 17:

DNA profiling (supplementary):

Disclosure of variation - Now that the sequence of the human genome is almost complete, the human genomics community is turning its attention towards what, genetically speaking, makes people different.
Saddam's DNA test a fast job (Wired News)
DNA profiling - Mallory, Lost on Everest (Nova Online)
also see the BIOG 1105-1106 DNA Lab page

Optional Supplementary Material:

Gene Therapy
RNA vs DNA
Origin of tRNA and rRNA

Genetic engineering:

Flower Power Takes on Land Mines (story with image)
Bacteria modified to help make missile fuel
Molecular Biology Tutorials
In Situ Hybridization diagram

Genome Mapping

For a basic overview of what genetic maps are and how they are produced, see The National Center for Biotechnology Information (NCBI) page "GENOME MAPPING: A GUIDE TO THE GENETIC HIGHWAY WE CALL THE HUMAN GENOME". The NCBI is a division of the National Library of Medicine (NLM) at the National Institutes of Health (NIH).

Follow the links below to information about efforts to map the genomes of these organisms and for articles about how the genetic maps produced are used.

human cow pig honey bee chimpanzee
chicken mouse rat dog pufferfish
fruit fly (Drosophila) kangaroo roundworm (C. elegans) microbes E. coli
salmonella cat Arabidopsis (related to mustard) Sea Squirt yeast (Saccharomyces)
rice corn banana mosquito tomato

DOE Joint Genome Institute Genome Portal

Genome project chimp dies unexpectedly

Compact chicken genome has less junk than mammals

© 2010 | BIOG 1105-1106